Team:TecMonterrey GDL/Parts

Parts

For E.coli

In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:

Strategy A : Part:BBa_K2760010 - Lactobachill Plan A (E. coli)

Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760002, BBa_K2760012, BBa_K2760029

Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill

Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760003, BBa_K2760012, BBa_K2760029

For L. rhamnosus GG

Strategy A: Part: BBa_K2760033

Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000,BBa_K2760002 BBa_K2760012, BBa_K2760029, BBa_K2760008

Plan B (L. rhamnosus): Part: BBa_K276004

Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760006, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000, BBa_K2760003, BBa_K2760012, BBa_K2760029, BBa_K2760008

Signaling peptides

For the excretion of recombinant proteins

For E.coli

Sp1-Nsp4 construct: BBa_K2760019 - Characterization construct for SGP1-NSP4 -

Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760000, BBa_K2760024, BBa_K2760012, BBa_B0015

BBa_K2760021 - SP2-Nsp2 Characterization construct

Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760004, BBa_K2760024, BBa_K2760012, BBa_B0015

BBa_K2760020 - Negative control for signal peptide characterization

Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760024, BBa_K2760012, BBa_B0015

BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)

Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760001, BBa_K2760024, BBa_K2760012, BBa_B0015

For L.rhamnosus

Sp1-Nsp4- Characterization construct Part: BBa_K2760030

Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760000, BBa_K2760029, BBa_K2760007

Sp2-Nsp2- Characterization Construct Part: BBa_K2760031

Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760004, BBa_K2760029, BBa_K2760007

Sp negative control construct: Part BBa_2760032

Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760029, BBa_K2760007

TecMonterrey_GDL